![]() Overall, results from this study demonstrated that non-mammalian sources of collagen could be used to confer bioactivity to PVDF, with comparable cell-material interactions and hemocompatibility to BVCOL. Also, cell surface expression of the leukocyte adhesion molecule was lower on HUVECs cultured on non-mammalian collagen surfaces than on BVCOL, which is an indication of lower pro-inflammatory response. Furthermore, both FSCOL and BFCOL had comparable anti-thrombogenic profiles to that of commercially available bovine collagen (BVCOL). The covalent immobilisation of fish scale-derived collagen (FSCOL) and bullfrog skin-derived collagen (BFCOL) onto the PVDF surface improves the attachment and proliferation of human umbilical vein endothelial cells (HUVECs). Results showed that Type I collagen was successfully extracted from fish scales and bullfrog skin. In this study, surface modification of PVDF using naturally derived non-mammalian collagen was accomplished via direct surface-initiated atom transfer radical polymerisation (SI-ATRP) to enhance its cytocompatibility and hemocompatibility. It’d be like a blender in there,” he said.Although polyvinylidene fluoride (PVDF) is non-toxic and stable in vivo, its hydrophobic surface has limited its bio-applications due to poor cell-material interaction and thrombus formation when used in blood contacting devices. There’s no seat belts, nothing to hold onto so if that bus ever hit something, you can imagine. ![]() “The windows weren’t big enough to get out of. ![]() … It looked like college kids made a party bus,” he said. Even renting a regular school bus with real seats would have been better, he said. He said he was concerned about safety because a party bus isn’t built to transport people 150 miles between cities. The only light came from the lights inside the three poles and there wasn’t any ventilation, he said. Passengers sat on benches placed around the sides of the bus’s interior and their luggage had to be placed in the open space in the middle of the bus, he said. He added that he watched an older man with a cane have difficulty getting onto the bus. He said they weren’t told about the change ahead of time, only finding out about it when they arrived at the transit center. It was a one-time mistake,” he said.ĭuluth resident Marty Anderson was “dumbfounded” when he dropped someone off to take Jefferson Lines to Minneapolis on Thursday. We will no longer be using that company as part of that situation. “We requested a picture of the bus they would be using, which was sent over as a regular motorcoach and they swapped it out and sent a different bus. Jefferson Lines turned to another company to provide a bus and checked that it had the necessary federal license, he said. The company had a situation arise this week in which the regular bus needed maintenance and it didn’t have a spare bus available for the Duluth-Minneapolis route because its backups were being used for charter trips, Pursey said. Jefferson Lines buses between Duluth and Minneapolis will return to normal beginning today, company spokesman Kevin Pursey said. “Who can’t not smile when you get on a party bus?” she asked. The driver, who didn’t give her last name, said the reaction to the party bus showing up had been good all day, with people grinning when they saw it pull up. If any of the Jefferson Lines passengers were surprised to learn their ride from Duluth to Minneapolis would be on a party bus - with a large sign on the back stating “Rent my party bus” - they didn’t show it as they hopped aboard with their luggage. Outside the school-type bus painted black, the bright lights of the three floor-to-ceiling poles used for dancing inside were only slightly visible through the bus’s tinted windows. Amid the city buses picking up passengers at the Duluth Transportation Center on Friday evening, a party bus driven by a woman named Sunshine pulled up to the curb to pick up its passengers. ![]()
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